TY - JOUR
T1 - Structured morphological modeling as a framework for rational strain design of Streptomyces species
AU - Celler, Katherine
AU - Picioreanu, Cristian
AU - Van Loosdrecht, Mark C.M.
AU - Van Wezel, Gilles P.
N1 - Generated from Scopus record by KAUST IRTS on 2022-09-13
PY - 2012/10/1
Y1 - 2012/10/1
N2 - Successful application of a computational model for rational design of industrial Streptomyces exploitation requires a better understanding of the relationship between morphology-dictated by micro-bial growth, branching, fragmentation and adhesion- and product formation. Here we review the state-of-the-art in modeling of growth and product formation by filamentous microorganisms and expand on existing models by combining a morphological and structural approach to realistically model and visualize a three-dimensional pellet. The objective is to provide a framework to study the effect of morphology and structure on natural product and enzyme formation and yield. Growth and development of the pellet occur via the processes of apical extension, branching and cross-wall formation. Oxygen is taken to be the limiting component, with the oxygen concentration at the tips regulating growth kinetics and the oxygen profile within the pellet affecting the probability of branching. Biological information regarding the processes of differentiation and branching in liquid cultures of the model organism Streptomyces coeli-color has been implemented. The model can be extended based on information gained in fermentation trials for different production strains, with the aim to provide a test drive for the fermentation process and to pre-assess the effect of different variables on productivity. This should aid in improving Streptomyces as a production platform in industrial biotechnology. © The Author(s) 2012.
AB - Successful application of a computational model for rational design of industrial Streptomyces exploitation requires a better understanding of the relationship between morphology-dictated by micro-bial growth, branching, fragmentation and adhesion- and product formation. Here we review the state-of-the-art in modeling of growth and product formation by filamentous microorganisms and expand on existing models by combining a morphological and structural approach to realistically model and visualize a three-dimensional pellet. The objective is to provide a framework to study the effect of morphology and structure on natural product and enzyme formation and yield. Growth and development of the pellet occur via the processes of apical extension, branching and cross-wall formation. Oxygen is taken to be the limiting component, with the oxygen concentration at the tips regulating growth kinetics and the oxygen profile within the pellet affecting the probability of branching. Biological information regarding the processes of differentiation and branching in liquid cultures of the model organism Streptomyces coeli-color has been implemented. The model can be extended based on information gained in fermentation trials for different production strains, with the aim to provide a test drive for the fermentation process and to pre-assess the effect of different variables on productivity. This should aid in improving Streptomyces as a production platform in industrial biotechnology. © The Author(s) 2012.
UR - http://link.springer.com/10.1007/s10482-012-9760-9
UR - http://www.scopus.com/inward/record.url?scp=84867578344&partnerID=8YFLogxK
U2 - 10.1007/s10482-012-9760-9
DO - 10.1007/s10482-012-9760-9
M3 - Article
SN - 0003-6072
VL - 102
SP - 409
EP - 423
JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
IS - 3
ER -