Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Jonas Kühn; Frédéric Montfort; Tristan Colomb; Benjamin Rappaz; Corinne Moratal; Nicolas Pavillon; Pierre Marquet; Christian Depeursinge

doi:10.1364/OL.34.000653

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Jonas Kühn^*, Frédéric Montfort, Tristan Colomb, Benjamin Rappaz, Corinne Moratal, Nicolas Pavillon, Pierre Marquet, Christian Depeursinge

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 μm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.

Original language	English (US)
Pages (from-to)	653-655
Number of pages	3
Journal	OPTICS LETTERS
Volume	34
Issue number	5
DOIs	https://doi.org/10.1364/OL.34.000653
State	Published - Mar 1 2009
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OL.34.000653

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AU - Montfort, Frédéric

AU - Colomb, Tristan

AU - Rappaz, Benjamin

AU - Moratal, Corinne

AU - Pavillon, Nicolas

AU - Marquet, Pierre

AU - Depeursinge, Christian

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AB - We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 μm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.

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Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Abstract

ASJC Scopus subject areas

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