Abstract
The aim of the present work was to investigate the potential utility of glycol chitosan coated liposomes as nasal vaccine delivery vehicle for eliciting viral specific humoral mucosal and cellular immune responses. Plasmid pRc/CMV-HBs(S) encapsulated liposomes were prepared by dehydration-rehydration method and subsequently coated with glycol chitosan by simple incubation method. Liposomes were then characterized for their size, surface charge, entrapment efficiency, and ability to protect encapsulated DNA against nuclease digestion and for their mucoadhesiveness. The liposomes were then administered to mice in order to study their feasibility as nasal vaccine carriers. The developed liposomes possessed +9.8 mV zeta potential and an average vesicle size less than 1 μm and entrapment efficiency of ∼53%. Following intranasal administration, glycol chitosan coated liposomes elicited humoral mucosal and cellular immune responses that were significant as compared to naked DNA justifying the potential advantage of mucosal vaccination in the production of local antibodies at the sites where pathogens enters the body. © 2008 Elsevier Ltd. All rights reserved.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2225-2233 |
| Number of pages | 9 |
| Journal | Vaccine |
| Volume | 26 |
| Issue number | 18 |
| DOIs | |
| State | Published - Apr 24 2008 |
| Externally published | Yes |
ASJC Scopus subject areas
- Infectious Diseases
- Molecular Medicine
- Public Health, Environmental and Occupational Health
- General Veterinary
- General Immunology and Microbiology
