TY - GEN
T1 - Survival of gfp-tagged antagonistic bacteria in the rhizosphere of tomato plants and their effects on the indigenous bacterial community
AU - Götz, Monika
AU - Gomes, Newton C.M.
AU - Dratwinski, Albert
AU - Costa, Rodrigo
AU - Berg, Gabriele
AU - Peixoto, Raquel
AU - Mendonça-Hagler, Leda
AU - Smalla, Kornelia
N1 - Generated from Scopus record by KAUST IRTS on 2021-05-05
PY - 2006/5/1
Y1 - 2006/5/1
N2 - The survival and colonization patterns of Pseudomonas putida PRD16 and Enterobacter cowanii PRF116 in the rhizosphere of greenhouse-grown tomato plants and the effects of their inoculation on the indigenous bacterial community were followed by selective plating, molecular fingerprinting, and confocal laser scanning microscopy (CLSM) over 3 weeks. Both strains, which showed in vitro antagonistic activity against Ralstonia solanacearum, were previously tagged with gfp. Seed and root inoculation were compared. Although plate counts decreased for both gfp-tagged antagonists, PRD16 showed a better survival in the rhizosphere of tomato roots independent of the inoculation method. Analysis of 16S rRNA gene fragments amplified from total community DNA by denaturing gradient gel electrophoresis and CLSM confirmed the decrease in the relative abundance of the inoculant strains. Pronounced differences in the Pseudomonas community patterns for plants inoculated with PRD16 compared to the control were detected 3 weeks after root inoculation, indicating a longer-lasting effect. Analysis by CLSM showed rather heterogeneous colonization patterns for both inoculant strains. In comparison with seed inoculation, root inoculation led to a much better colonization as evidenced by all three methods. The colonization patterns observed by CLSM provide important information on the sampling strategy required for monitoring inoculant strains in the rhizosphere. © 2006 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
AB - The survival and colonization patterns of Pseudomonas putida PRD16 and Enterobacter cowanii PRF116 in the rhizosphere of greenhouse-grown tomato plants and the effects of their inoculation on the indigenous bacterial community were followed by selective plating, molecular fingerprinting, and confocal laser scanning microscopy (CLSM) over 3 weeks. Both strains, which showed in vitro antagonistic activity against Ralstonia solanacearum, were previously tagged with gfp. Seed and root inoculation were compared. Although plate counts decreased for both gfp-tagged antagonists, PRD16 showed a better survival in the rhizosphere of tomato roots independent of the inoculation method. Analysis of 16S rRNA gene fragments amplified from total community DNA by denaturing gradient gel electrophoresis and CLSM confirmed the decrease in the relative abundance of the inoculant strains. Pronounced differences in the Pseudomonas community patterns for plants inoculated with PRD16 compared to the control were detected 3 weeks after root inoculation, indicating a longer-lasting effect. Analysis by CLSM showed rather heterogeneous colonization patterns for both inoculant strains. In comparison with seed inoculation, root inoculation led to a much better colonization as evidenced by all three methods. The colonization patterns observed by CLSM provide important information on the sampling strategy required for monitoring inoculant strains in the rhizosphere. © 2006 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
UR - https://academic.oup.com/femsec/article-lookup/doi/10.1111/j.1574-6941.2006.00093.x
UR - http://www.scopus.com/inward/record.url?scp=33646014149&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6941.2006.00093.x
DO - 10.1111/j.1574-6941.2006.00093.x
M3 - Conference contribution
SP - 207
EP - 218
BT - FEMS Microbiology Ecology
ER -