Symbiotic Photosynthetic Oxygenation within 3D-Bioprinted Vascularized Tissues

Sushila Maharjan, Jacqueline Alva, Cassandra Cámara, Andrés G. Rubio, David Hernández, Clément Delavaux, Erandy Correa, Mariana D. Romo, Diana Bonilla, Mille Luis Santiago, Wanlu Li, Feng Cheng, Guoliang Ying, Yu Shrike Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

In this study, we present the photosynthetic oxygen (O2) supply to mammalian cells within a volumetric extracellular matrix-like construct, whereby a three-dimensional (3D)-bioprinted fugitive pattern encapsulating unicellular green algae, Chlamydomonas reinhardtii, served as a natural photosynthetic O2 generator. The presence of bioprinted C. reinhardtii enhanced the viability and functionality of mammalian cells while reducing the hypoxic conditions within the tissues. We were able to subsequently endothelialize the hollow perfusable microchannels formed after enzymatic removal of the bioprinted C. reinhardtii-laden patterns from the matrices following the initial oxygenation period to obtain biologically relevant vascularized mammalian tissue constructs. The feasibility of co-culture of C. reinhardtii with human cells, the printability and the enzymatic degradability of the fugitive bioink, and the exploration of C. reinhardtii as a natural, eco-friendly, cost-effective, and sustainable source of O2 would likely promote the development of engineered tissues, tissue models, and food for various applications.

Original languageEnglish (US)
Pages (from-to)217-240
Number of pages24
JournalMatter
Volume4
Issue number1
DOIs
StatePublished - Jan 6 2021

Keywords

  • algae
  • biofabrication
  • bioink
  • bioprinting
  • Chlamydomonas reinhardtii
  • MAP6: Development
  • oxygenation
  • photosynthesis
  • regenerative medicine
  • tissue engineering
  • vascularization

ASJC Scopus subject areas

  • General Materials Science

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