Synthesis and Evaluation of Novel Carboxamides Capable of Causing Centrosome Declustering and Apoptosis in Breast Cancer Cells

Usama B. Farrukh, Aishah Bilal, Huda Zahid, Maheen Iqbal, Safia Manzoor, Farhat Firdous, Muhammad Furqan, Muhammad Azeem, Abdul Hamid Emwas, Meshari Alazmi, Xin Gao, Rahman S.Z. Saleem*, Amir Faisal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The fragility of cancer cells at the time of mitosis has served as an important target for the development of many successful chemotherapeutic agents. Many cancers cells have supernumerary centrosomes that they cluster during mitosis to form bipolar spindles. Inhibition of centrosome clustering in these cells results in multipolar spindle formation and apoptotic cell death, providing an opportunity to selectively target a subset of cancers with centrosome amplification. In the current work, we report synthesis of 29 novel tethered biaryls and biological evaluation of their ability to inhibit centrosome clustering in breast cancer cells (BT-549). We have identified N-benzhydryl-5-nitrofuran-2-carboxamide (5 h) as a centrosome declustering compound. 5 h has potent antiproliferative activity in centrosome amplified BT-549 cells with GI50 value of 1.81±0.19 μM (n=2). Treatment of BT-549 cells with 5 h causes centrosome declustering resulting in mitotic arrest due to multipolar spindle formation and misaligned chromosomes which ultimately leads to apoptotic cell death.

Original languageEnglish (US)
Article numbere202104218
JournalChemistrySelect
Volume7
Issue number15
DOIs
StatePublished - Apr 21 2022

Keywords

  • Antitumor agents
  • Apoptosis
  • Carboxamide
  • Centrosome declustering
  • Nitrofuran

ASJC Scopus subject areas

  • General Chemistry

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