Targeted and Random Transposon-Assisted Single-Copy Transgene Insertion in C. elegans

Sonia El Mouridi, Christian Frøkjær-Jensen

Research output: Chapter in Book/Report/Conference proceedingChapter


Transgenesis in model organisms is an essential tool for determining the function of protein-coding genes and non-coding regulatory regions. In Caenorhabditis elegans, injected DNA can be propagated as multicopy extra-chromosomal arrays, but transgenes in arrays are frequently mosaic, over-expressed in some tissues, and silenced in the germline. Here, we describe methods to insert single-copy transgenes into specific genomic locations (MosSCI) or random locations (miniMos) using Mos1 transposons. Single-copy insertions allow expression at endogenous levels, expression in the germline, and identification of active and repressed regions of the genome.
Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherSpringer US
Number of pages18
ISBN (Print)9781071621806
StatePublished - Mar 24 2022


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