@article{fdd41c78472c4c888b5b672781d441f2,
title = "Targeted gene deletions in C. elegans using transposon excision",
abstract = "We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.",
author = "Christian Fr{\o}kj{\ae}r-Jensen and Davis, {M. Wayne} and Gunther Hollopeter and Jon Taylor and Harris, {Todd W.} and Paola Nix and Rachel Lofgren and Michael Prestgard-Duke and Michael Bastiani and Moerman, {Donald G.} and Jorgensen, {Erik M.}",
note = "Funding Information: We thank L. Segalat (Centre National de la Recherche Scientifique, Lyon, France) and members of the NemaGENETAG consortium for Mos1 strains. C.F.-J. is funded by a fellowship from the Lundbeck Foundation and G.H. by a Jane Coffin Childs fellowship funded by Howard Hughes Medical Institute. M.B. is funded by a McKnight Grant. D.G.M. is funded by Genome Canada and Genome British Columbia.",
year = "2010",
month = jun,
doi = "10.1038/nmeth.1454",
language = "English (US)",
volume = "7",
pages = "451--453",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "6",
}