Tbx2 and Tbx3 regulate the dynamics of cell proliferation during heart remodeling

Inês Ribeiro, Yasuhiko Kawakami, Dirk Büscher, Ángel Raya, Joaquín Rodriguez-León, Masanobu Morita, Concepción Rodríguez Esteban, Juan Carlos Izpisúa Belmonte

Research output: Contribution to journalArticlepeer-review

81 Scopus citations


Background. The heart forms from a linear tube that is subject to complex remodeling during embryonic development. Hallmarks of this remodeling are the looping of the heart tube and the regionalization into chamber and non-chamber myocardium. Cardiomyocytes in the future chamber myocardium acquire different cellular and physiological characteristics through activation of a chamber-specific genetic program, which is in part mediated by T-box genes. Methodology Principal Finding. We characterize two new zebrafish T-box transcription factors, tbx3b and tbx2a, and analyze their role during the development of the atrioventricular canal. Loss- and gain-of-function analyses demonstrate that tbx3band tbx2a are necessary to repress the chamber-genetic program in the non-chamber myocardium. We also show that tbx3b and tbx2a are required to control cell proliferation in the atrioventricular canal and that misregulation of cell proliferation in the heart tube influences looping. Furthermore, we characterize the heart phenotype of a novel Tbx3 mutation in mice and show that both the control of cell proliferation and the repression of chamber-specific genetic program in the non-chamber myocardium are conserved roles of Tbx3 in this species. Conclusions/Significance. Taken together, our results uncover an evolutionarily conserved role of Tbx2/3 transcription factors during remodeling of the heart myocardium and highlight the importance of controlling cell proliferation as a driving force of morphogenesis.

Original languageEnglish (US)
Article numbere398
JournalPloS one
Issue number4
StatePublished - Apr 25 2007
Externally publishedYes

ASJC Scopus subject areas

  • General


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