The dynamic multisite interactions between two intrinsically disordered proteins

Shaowen Wu, Dongdong Wang, Jin Liu, Yitao Feng, Jingwei Weng, Yu Li, Xin Gao, Jianwei Liu, Wenning Wang

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Protein interactions involving intrinsically disordered proteins (IDPs) comprise a variety of binding modes, from the well characterized folding upon binding to dynamic fuzzy complex. To date, most studies concern the binding of an IDP to a structured protein, while the Interaction between two IDPs is poorly understood. In this study, we combined NMR, smFRET, and molecular dynamics (MD) simulation to characterize the interaction between two IDPs, the C-terminal domain (CTD) of protein 4.1G and the nuclear mitotic apparatus (NuMA) protein. It is revealed that CTD and NuMA form a fuzzy complex with remaining structural disorder. Multiple binding sites on both proteins were identified by MD and mutagenesis studies. Our study provides an atomic scenario in which two IDPs bearing multiple binding sites interact with each other in dynamic equilibrium. The combined approach employed here could be widely applicable for investigating IDPs and their dynamic interactions.
Original languageEnglish (US)
Pages (from-to)7515-7519
Number of pages5
JournalAngewandte Chemie International Edition
Issue number26
StatePublished - May 24 2017


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