The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip

Ran Lin; Donald C. Chang; Yi-Kuen Lee

doi:10.1109/nems.2012.6196744

The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip

Ran Lin, Donald C. Chang, Yi-Kuen Lee

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Electroendocytosis (EED) is a pulsed-electric-field (PEF) induced endocytosis, facilitating cells uptake molecules through nanometer-sized EED vesicles. We herein investigate the effect of a chemical inhibitor, Cytochalasin D (CD) on the actin-filaments (F-Actin) behavior of single-cell EED. The CD concentration (C CD) can control the depolymerization of F-actin. A multi-chamber micro cell chip was fabricated to study the EED under different conditions. Large-scale single-cell data demonstrated EED highly depends on both electric field and C CD. © 2012 IEEE.

Original language	English (US)
Title of host publication	2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	150-153
Number of pages	4
ISBN (Print)	9781467311243
DOIs	https://doi.org/10.1109/nems.2012.6196744
State	Published - Mar 2012
Externally published	Yes

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Lin, R., Chang, D. C., & Lee, Y.-K. (2012). The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) (pp. 150-153). Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/nems.2012.6196744

@inproceedings{bcb7c14acccd49ee8a525d9e8e279b62,

title = "The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip",

abstract = "Electroendocytosis (EED) is a pulsed-electric-field (PEF) induced endocytosis, facilitating cells uptake molecules through nanometer-sized EED vesicles. We herein investigate the effect of a chemical inhibitor, Cytochalasin D (CD) on the actin-filaments (F-Actin) behavior of single-cell EED. The CD concentration (C CD) can control the depolymerization of F-actin. A multi-chamber micro cell chip was fabricated to study the EED under different conditions. Large-scale single-cell data demonstrated EED highly depends on both electric field and C CD. {\textcopyright} 2012 IEEE.",

author = "Ran Lin and Chang, {Donald C.} and Yi-Kuen Lee",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): SA-C0040, UK-COOI6 Acknowledgements: The research was sponsored by a grant from Hong Kong Research Grants Council (Grant Ref. No. 615907) and by an award from the King Abdullah University of Science and Technology (KAUST Award No. SA-C0040/UK-COOI6). This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2012",

month = mar,

doi = "10.1109/nems.2012.6196744",

language = "English (US)",

isbn = "9781467311243",

pages = "150--153",

booktitle = "2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)",

publisher = "Institute of Electrical and Electronics Engineers (IEEE)",

}

The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip. / Lin, Ran; Chang, Donald C.; Lee, Yi-Kuen.
2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). Institute of Electrical and Electronics Engineers (IEEE), 2012. p. 150-153.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip

AU - Lin, Ran

AU - Chang, Donald C.

AU - Lee, Yi-Kuen

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): SA-C0040, UK-COOI6 Acknowledgements: The research was sponsored by a grant from Hong Kong Research Grants Council (Grant Ref. No. 615907) and by an award from the King Abdullah University of Science and Technology (KAUST Award No. SA-C0040/UK-COOI6). This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2012/3

Y1 - 2012/3

N2 - Electroendocytosis (EED) is a pulsed-electric-field (PEF) induced endocytosis, facilitating cells uptake molecules through nanometer-sized EED vesicles. We herein investigate the effect of a chemical inhibitor, Cytochalasin D (CD) on the actin-filaments (F-Actin) behavior of single-cell EED. The CD concentration (C CD) can control the depolymerization of F-actin. A multi-chamber micro cell chip was fabricated to study the EED under different conditions. Large-scale single-cell data demonstrated EED highly depends on both electric field and C CD. © 2012 IEEE.

AB - Electroendocytosis (EED) is a pulsed-electric-field (PEF) induced endocytosis, facilitating cells uptake molecules through nanometer-sized EED vesicles. We herein investigate the effect of a chemical inhibitor, Cytochalasin D (CD) on the actin-filaments (F-Actin) behavior of single-cell EED. The CD concentration (C CD) can control the depolymerization of F-actin. A multi-chamber micro cell chip was fabricated to study the EED under different conditions. Large-scale single-cell data demonstrated EED highly depends on both electric field and C CD. © 2012 IEEE.

UR - http://hdl.handle.net/10754/600264

UR - http://ieeexplore.ieee.org/document/6196744/

UR - http://www.scopus.com/inward/record.url?scp=84861542073&partnerID=8YFLogxK

U2 - 10.1109/nems.2012.6196744

DO - 10.1109/nems.2012.6196744

M3 - Conference contribution

SN - 9781467311243

SP - 150

EP - 153

BT - 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

PB - Institute of Electrical and Electronics Engineers (IEEE)

ER -

The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip

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