The independent domestication of timopheev’s wheat: Insights from haplotype analysis of the brittle rachis 1 (btr1‐a) gene

Moran Nave, Mihriban Taş, John Raupp, Vijay K. Tiwari, Hakan Ozkan, Jesse Poland, Iago Hale, Takao Komatsuda, Assaf Distelfeld

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Triticum turgidum and T. timopheevii are two tetraploid wheat species sharing T. urartu as a common ancestor, and domesticated accessions from both of these allopolyploids exhibit nonbrittle rachis (i.e., nonshattering spikes). We previously described the loss‐of‐function mutations in the Brittle Rachis 1 genes BTR1‐A and BTR1‐B in the A and B subgenomes, respectively, that are responsible for this most visible domestication trait in T. turgidum. Resequencing of a large panel of wild and domesticated T. turgidum accessions subsequently led to the identification of the two progenitor haplotypes of the btr1‐A and btr1‐B domesticated alleles. Here, we extended the haplotype analysis to other T. turgidum subspecies and to the BTR1 homologues in the related T. timopheevii species. Our results showed that all the domesticated wheat subspecies within T. turgidum share common BTR1‐A and BTR1‐B haplotypes, confirming their common origin. In T. timopheevii, however, we identified a novel loss‐of‐function btr1‐A allele underlying a partially brittle spike phenotype. This novel recessive allele appeared fixed within the pool of domesticated Timopheev’s wheat but was also carried by one wild timopheevii accession exhibiting partial brittleness. The promoter region for BTR1‐B could not be amplified in any T. timopheevii accessions with any T. turgidum primer combination, exemplifying the gene‐level distance between the two species. Altogether, our results support the concept of independent domestication processes for the two polyploid, wheat‐related species.
Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
Issue number3
StatePublished - Mar 1 2021
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)


Dive into the research topics of 'The independent domestication of timopheev’s wheat: Insights from haplotype analysis of the brittle rachis 1 (btr1‐a) gene'. Together they form a unique fingerprint.

Cite this