The MAP kinase substrate MKS1 is a regulator of plant defense responses

Erik Andreasson; Thomas Jenkins; Peter Brodersen; Stephan Thorgrimsen; Nikolaj H.T. Petersen; Shijiang Zhu; Jin Long Qiu; Pernille Micheelsen; Anne Rocher; Morten Petersen; Mari Anne Newman; Henrik Björn Nielsen; Heribert Hirt; Imre Somssich; Ole Mattsson; John Mundy

doi:10.1038/sj.emboj.7600737

The MAP kinase substrate MKS1 is a regulator of plant defense responses

Erik Andreasson, Thomas Jenkins, Peter Brodersen, Stephan Thorgrimsen, Nikolaj H.T. Petersen, Shijiang Zhu, Jin Long Qiu, Pernille Micheelsen, Anne Rocher, Morten Petersen, Mari Anne Newman, Henrik Björn Nielsen, Heribert Hirt, Imre Somssich, Ole Mattsson, John Mundy^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

422 Scopus citations

Abstract

Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.

Original language	English (US)
Pages (from-to)	2579-2589
Number of pages	11
Journal	EMBO JOURNAL
Volume	24
Issue number	14
DOIs	https://doi.org/10.1038/sj.emboj.7600737
State	Published - Jul 20 2005
Externally published	Yes

Keywords

Disease resistance
Phosphoserine
Protein kinase

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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Andreasson, E., Jenkins, T., Brodersen, P., Thorgrimsen, S., Petersen, N. H. T., Zhu, S., Qiu, J. L., Micheelsen, P., Rocher, A., Petersen, M., Newman, M. A., Nielsen, H. B., Hirt, H., Somssich, I., Mattsson, O., & Mundy, J. (2005). The MAP kinase substrate MKS1 is a regulator of plant defense responses. EMBO JOURNAL, 24(14), 2579-2589. https://doi.org/10.1038/sj.emboj.7600737

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title = "The MAP kinase substrate MKS1 is a regulator of plant defense responses",

abstract = "Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.",

keywords = "Disease resistance, Phosphoserine, Protein kinase",

author = "Erik Andreasson and Thomas Jenkins and Peter Brodersen and Stephan Thorgrimsen and Petersen, {Nikolaj H.T.} and Shijiang Zhu and Qiu, {Jin Long} and Pernille Micheelsen and Anne Rocher and Morten Petersen and Newman, {Mari Anne} and Nielsen, {Henrik Bj{\"o}rn} and Heribert Hirt and Imre Somssich and Ole Mattsson and John Mundy",

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doi = "10.1038/sj.emboj.7600737",

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T1 - The MAP kinase substrate MKS1 is a regulator of plant defense responses

AU - Andreasson, Erik

AU - Jenkins, Thomas

AU - Brodersen, Peter

AU - Thorgrimsen, Stephan

AU - Petersen, Nikolaj H.T.

AU - Zhu, Shijiang

AU - Qiu, Jin Long

AU - Micheelsen, Pernille

AU - Rocher, Anne

AU - Petersen, Morten

AU - Newman, Mari Anne

AU - Nielsen, Henrik Björn

AU - Hirt, Heribert

AU - Somssich, Imre

AU - Mattsson, Ole

AU - Mundy, John

PY - 2005/7/20

Y1 - 2005/7/20

N2 - Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.

AB - Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.

KW - Disease resistance

KW - Phosphoserine

KW - Protein kinase

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ER -

The MAP kinase substrate MKS1 is a regulator of plant defense responses

Abstract

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