TY - JOUR
T1 - The membrane bioreactor: A novel tool to grow anammox bacteria as free cells
AU - Van Der Star, Wouter R.L.
AU - Miclea, Andreea I.
AU - Van Dongen, Udo G.J.M.
AU - Muyzer, Gerard
AU - Picioreanu, Cristian
AU - Van Loosdrecht, Mark C.M.
N1 - Generated from Scopus record by KAUST IRTS on 2022-09-13
PY - 2008/10/1
Y1 - 2008/10/1
N2 - In a membrane bioreactor (MBR), fast growth of anammox bacteria was achieved with a sludge residence time (SRT) of 12 days. This relatively short SRT resulted in a - for anammox bacteria - unprecedented purity of the enrichment of 97.6%. The absence of a selective pressure for settling, and dedicated cultivation conditions led to growth in suspension as free cells and the complete absence of flocs or granules. Fast growth, low levels of calcium and magnesium, and possibly the presence of yeast extract and a low shear stress are critical for the obtainment of a completely suspended culture consisting of free anammox cells. During cultivation, a population shift was observed from Candidatus "Brocadia" to Candidatus "Kuenenia stuttgartiensis." It is hypothesized that the reason for this shift is the higher affinity for nitrite of "Kuenenia." The production of anammox bacteria in suspension with high purity and productivity makes the MBR a promising tool for the cultivation and study of anammox bacteria. © 2008 Wiley Periodicals, Inc.
AB - In a membrane bioreactor (MBR), fast growth of anammox bacteria was achieved with a sludge residence time (SRT) of 12 days. This relatively short SRT resulted in a - for anammox bacteria - unprecedented purity of the enrichment of 97.6%. The absence of a selective pressure for settling, and dedicated cultivation conditions led to growth in suspension as free cells and the complete absence of flocs or granules. Fast growth, low levels of calcium and magnesium, and possibly the presence of yeast extract and a low shear stress are critical for the obtainment of a completely suspended culture consisting of free anammox cells. During cultivation, a population shift was observed from Candidatus "Brocadia" to Candidatus "Kuenenia stuttgartiensis." It is hypothesized that the reason for this shift is the higher affinity for nitrite of "Kuenenia." The production of anammox bacteria in suspension with high purity and productivity makes the MBR a promising tool for the cultivation and study of anammox bacteria. © 2008 Wiley Periodicals, Inc.
UR - https://onlinelibrary.wiley.com/doi/10.1002/bit.21891
UR - http://www.scopus.com/inward/record.url?scp=51649104986&partnerID=8YFLogxK
U2 - 10.1002/bit.21891
DO - 10.1002/bit.21891
M3 - Article
SN - 1097-0290
VL - 101
SP - 286
EP - 294
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 2
ER -