The molecular structure of the Corynebacterium glutamicum threonine synthase gene

K. ‐S Han; J. A.C. Archer; A. J. Sinskey

doi:10.1111/j.1365-2958.1990.tb00546.x

The molecular structure of the Corynebacterium glutamicum threonine synthase gene

K. ‐S Han, J. A.C. Archer, A. J. Sinskey^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The minimal region encoding the Corynebacterium glutamicum threonine synthase structural gene and its promoter was mapped by deletion analysis and complementation of the C. glutamicum thrC allele to a 1.6kb region of the recombinant plasmid pFS80. The nucleotide sequence of this and flanking DNA was determined. The transcription and translation start points were identified by S1 mapping analysis and amino‐terminal protein sequencing, respectively. The thrC gene encodes a 54481‐Dalton polypeptide product. Translation of the thrC mRNA initiates only six nucleotides downstream from transcription. The length of the mRNA transcript is consistent with a single gene transcription unit. The C. glutamicum thrC gene is expressed independently of the other threonine‐specific genes hom and thrB.

Original language	English (US)
Pages (from-to)	1693-1702
Number of pages	10
Journal	Molecular Microbiology
Volume	4
Issue number	10
DOIs	https://doi.org/10.1111/j.1365-2958.1990.tb00546.x
State	Published - Oct 1990
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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abstract = "The minimal region encoding the Corynebacterium glutamicum threonine synthase structural gene and its promoter was mapped by deletion analysis and complementation of the C. glutamicum thrC allele to a 1.6kb region of the recombinant plasmid pFS80. The nucleotide sequence of this and flanking DNA was determined. The transcription and translation start points were identified by S1 mapping analysis and amino‐terminal protein sequencing, respectively. The thrC gene encodes a 54481‐Dalton polypeptide product. Translation of the thrC mRNA initiates only six nucleotides downstream from transcription. The length of the mRNA transcript is consistent with a single gene transcription unit. The C. glutamicum thrC gene is expressed independently of the other threonine‐specific genes hom and thrB.",

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AB - The minimal region encoding the Corynebacterium glutamicum threonine synthase structural gene and its promoter was mapped by deletion analysis and complementation of the C. glutamicum thrC allele to a 1.6kb region of the recombinant plasmid pFS80. The nucleotide sequence of this and flanking DNA was determined. The transcription and translation start points were identified by S1 mapping analysis and amino‐terminal protein sequencing, respectively. The thrC gene encodes a 54481‐Dalton polypeptide product. Translation of the thrC mRNA initiates only six nucleotides downstream from transcription. The length of the mRNA transcript is consistent with a single gene transcription unit. The C. glutamicum thrC gene is expressed independently of the other threonine‐specific genes hom and thrB.

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The molecular structure of the Corynebacterium glutamicum threonine synthase gene

Abstract

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