The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner

Claudia Jonak; Aniko Páy; Laszlo Bögre; Heribert Hirt; Erwin Heberle-Bors

doi:10.1046/j.1365-313X.1993.03040611.x

The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner

Claudia Jonak, Aniko Páy, Laszlo Bögre, Heribert Hirt^*, Erwin Heberle-Bors

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

99 Scopus citations

Abstract

In animals, MAP kinase plays a key role in growth factor-stimulated signalling and in mitosis. The isolation of a Medicago sativa cDNA clone MsK7 which shows 52% identity to animal MAP kinases is reported. The deduced protein sequence shows all the important structural features of MAP kinases and also contains the highly conserved Thr-183 and Tyr-185 residues. Northern analysis of synchronized alfalfa cells showed that the MsK7 kinase gene is expressed at low levels in G1 phase but at higher levels in S and G2 phases of the cell cycle. In the plant, only stems and roots were found to contain MAP kinase MsK7 mRNA. Southern and PCR analyses indicated that alfalfa contains at least four highly related MAP kinase genes.

Original language	English (US)
Pages (from-to)	611-617
Number of pages	7
Journal	Plant Journal
Volume	3
Issue number	4
DOIs	https://doi.org/10.1046/j.1365-313X.1993.03040611.x
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Genetics
Plant Science
Cell Biology

Access to Document

10.1046/j.1365-313X.1993.03040611.x

Cite this

@article{97c803e07f3d4f599e4d5630323ada5e,

title = "The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner",

abstract = "In animals, MAP kinase plays a key role in growth factor-stimulated signalling and in mitosis. The isolation of a Medicago sativa cDNA clone MsK7 which shows 52% identity to animal MAP kinases is reported. The deduced protein sequence shows all the important structural features of MAP kinases and also contains the highly conserved Thr-183 and Tyr-185 residues. Northern analysis of synchronized alfalfa cells showed that the MsK7 kinase gene is expressed at low levels in G1 phase but at higher levels in S and G2 phases of the cell cycle. In the plant, only stems and roots were found to contain MAP kinase MsK7 mRNA. Southern and PCR analyses indicated that alfalfa contains at least four highly related MAP kinase genes.",

author = "Claudia Jonak and Aniko P{\'a}y and Laszlo B{\"o}gre and Heribert Hirt and Erwin Heberle-Bors",

year = "1993",

doi = "10.1046/j.1365-313X.1993.03040611.x",

language = "English (US)",

volume = "3",

pages = "611--617",

journal = "Plant Journal",

issn = "0960-7412",

publisher = "Wiley",

number = "4",

}

TY - JOUR

T1 - The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner

AU - Jonak, Claudia

AU - Páy, Aniko

AU - Bögre, Laszlo

AU - Hirt, Heribert

AU - Heberle-Bors, Erwin

PY - 1993

Y1 - 1993

N2 - In animals, MAP kinase plays a key role in growth factor-stimulated signalling and in mitosis. The isolation of a Medicago sativa cDNA clone MsK7 which shows 52% identity to animal MAP kinases is reported. The deduced protein sequence shows all the important structural features of MAP kinases and also contains the highly conserved Thr-183 and Tyr-185 residues. Northern analysis of synchronized alfalfa cells showed that the MsK7 kinase gene is expressed at low levels in G1 phase but at higher levels in S and G2 phases of the cell cycle. In the plant, only stems and roots were found to contain MAP kinase MsK7 mRNA. Southern and PCR analyses indicated that alfalfa contains at least four highly related MAP kinase genes.

AB - In animals, MAP kinase plays a key role in growth factor-stimulated signalling and in mitosis. The isolation of a Medicago sativa cDNA clone MsK7 which shows 52% identity to animal MAP kinases is reported. The deduced protein sequence shows all the important structural features of MAP kinases and also contains the highly conserved Thr-183 and Tyr-185 residues. Northern analysis of synchronized alfalfa cells showed that the MsK7 kinase gene is expressed at low levels in G1 phase but at higher levels in S and G2 phases of the cell cycle. In the plant, only stems and roots were found to contain MAP kinase MsK7 mRNA. Southern and PCR analyses indicated that alfalfa contains at least four highly related MAP kinase genes.

UR - http://www.scopus.com/inward/record.url?scp=0027587287&partnerID=8YFLogxK

U2 - 10.1046/j.1365-313X.1993.03040611.x

DO - 10.1046/j.1365-313X.1993.03040611.x

M3 - Article

C2 - 8220466

AN - SCOPUS:0027587287

SN - 0960-7412

VL - 3

SP - 611

EP - 617

JO - Plant Journal

JF - Plant Journal

IS - 4

ER -

The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this