TY - JOUR
T1 - The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds
AU - Gerhardt, Lutz Christian
AU - Widdows, Kate L.
AU - Erol, Melek M.
AU - Burch, Charles W.
AU - Sanz-Herrera, José A.
AU - Ochoa, Ignacio
AU - Stämpfli, Rolf
AU - Roqan, Iman S.
AU - Gabe, Simon M.
AU - Ansari, Tahera I.
AU - Boccaccini, Aldo R.
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors acknowledge the financial support of King Abdullah University of Science and Technology (KAUST). Special thanks are due to Dr. L Serra (Roche, Germany), Dr. J. Lackey (Insight Biotechnology, UK), C. Akufo-Addo (LGC Standards, UK), Dr. M. Ardakani, E. Ware, R. Chater (all Imperial College London) for technical support and helpful discussions. The authors also acknowledge Prof. W. Stark and D. Mohn (ETH Zurich) for providing the nano-sized BG used.
PY - 2011/6
Y1 - 2011/6
N2 - The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.
AB - The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.
UR - http://hdl.handle.net/10754/561783
UR - https://linkinghub.elsevier.com/retrieve/pii/S0142961211001840
UR - http://www.scopus.com/inward/record.url?scp=79953292885&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2011.02.032
DO - 10.1016/j.biomaterials.2011.02.032
M3 - Article
C2 - 21411138
SN - 0142-9612
VL - 32
SP - 4096
EP - 4108
JO - Biomaterials
JF - Biomaterials
IS - 17
ER -