The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

Lutz Christian Gerhardt; Kate L. Widdows; Melek M. Erol; Charles W. Burch; José A. Sanz-Herrera; Ignacio Ochoa; Rolf Stämpfli; Iman S. Roqan; Simon M. Gabe; Tahera I. Ansari; Aldo R. Boccaccini

doi:10.1016/j.biomaterials.2011.02.032

The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

Lutz Christian Gerhardt, Kate L. Widdows, Melek M. Erol, Charles W. Burch, José A. Sanz-Herrera, Ignacio Ochoa, Rolf Stämpfli, Iman S. Roqan, Simon M. Gabe, Tahera I. Ansari, Aldo R. Boccaccini

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181 Scopus citations

Abstract

The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.

Original language	English (US)
Pages (from-to)	4096-4108
Number of pages	13
Journal	Biomaterials
Volume	32
Issue number	17
DOIs	https://doi.org/10.1016/j.biomaterials.2011.02.032
State	Published - Jun 2011

ASJC Scopus subject areas

Biomaterials
Bioengineering
Mechanics of Materials
Ceramics and Composites
Biophysics

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10.1016/j.biomaterials.2011.02.032

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@article{8c5fb820eabe4285b62b44c7d5092882,

title = "The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds",

abstract = "The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. {\textcopyright} 2011 Elsevier Ltd.",

author = "Gerhardt, {Lutz Christian} and Widdows, {Kate L.} and Erol, {Melek M.} and Burch, {Charles W.} and Sanz-Herrera, {Jos{\'e} A.} and Ignacio Ochoa and Rolf St{\"a}mpfli and Roqan, {Iman S.} and Gabe, {Simon M.} and Ansari, {Tahera I.} and Boccaccini, {Aldo R.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors acknowledge the financial support of King Abdullah University of Science and Technology (KAUST). Special thanks are due to Dr. L Serra (Roche, Germany), Dr. J. Lackey (Insight Biotechnology, UK), C. Akufo-Addo (LGC Standards, UK), Dr. M. Ardakani, E. Ware, R. Chater (all Imperial College London) for technical support and helpful discussions. The authors also acknowledge Prof. W. Stark and D. Mohn (ETH Zurich) for providing the nano-sized BG used.",

year = "2011",

month = jun,

doi = "10.1016/j.biomaterials.2011.02.032",

language = "English (US)",

volume = "32",

pages = "4096--4108",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier Ltd",

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T1 - The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

AU - Gerhardt, Lutz Christian

AU - Widdows, Kate L.

AU - Erol, Melek M.

AU - Burch, Charles W.

AU - Sanz-Herrera, José A.

AU - Ochoa, Ignacio

AU - Stämpfli, Rolf

AU - Roqan, Iman S.

AU - Gabe, Simon M.

AU - Ansari, Tahera I.

AU - Boccaccini, Aldo R.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors acknowledge the financial support of King Abdullah University of Science and Technology (KAUST). Special thanks are due to Dr. L Serra (Roche, Germany), Dr. J. Lackey (Insight Biotechnology, UK), C. Akufo-Addo (LGC Standards, UK), Dr. M. Ardakani, E. Ware, R. Chater (all Imperial College London) for technical support and helpful discussions. The authors also acknowledge Prof. W. Stark and D. Mohn (ETH Zurich) for providing the nano-sized BG used.

PY - 2011/6

Y1 - 2011/6

N2 - The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.

AB - The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.

UR - http://hdl.handle.net/10754/561783

UR - https://linkinghub.elsevier.com/retrieve/pii/S0142961211001840

UR - http://www.scopus.com/inward/record.url?scp=79953292885&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2011.02.032

DO - 10.1016/j.biomaterials.2011.02.032

M3 - Article

C2 - 21411138

SN - 0142-9612

VL - 32

SP - 4096

EP - 4108

JO - Biomaterials

JF - Biomaterials

IS - 17

ER -

The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

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