TY - JOUR
T1 - The RETINOBLASTOMA-RELATED gene regulates stem cell maintenance in Arabidopsis roots
AU - Wildwater, Marjolein
AU - Campilho, Ana
AU - Perez-Perez, Jose Manuel
AU - Heidstra, Renze
AU - Blilou, Ikram
AU - Korthout, Henrie
AU - Chatterjee, Jayanta
AU - Mariconti, Luisa
AU - Gruissem, Wilhelm
AU - Scheres, Ben
PY - 2005/12/29
Y1 - 2005/12/29
N2 - The maintenance of stem cells in defined locations is crucial for all multicellular organisms. Although intrinsic factors and signals for stem cell fate have been identified in several species, it has remained unclear how these connect to the ability to reenter the cell cycle that is one of the defining properties of stem cells. We show that local reduction of expression of the RETINOBLASTOMA-RELATED (RBR) gene in Arabidopsis roots increases the amount of stem cells without affecting cell cycle duration in mitotically active cells. Conversely, induced RBR overexpression dissipates stem cells prior to arresting other mitotic cells. Overexpression of D cyclins, KIP-related proteins, and E2F factors also affects root stem cell pool size, and genetic interactions suggest that these factors function in a canonical RBR pathway to regulate somatic stem cells. Expression analysis and genetic interactions position RBR-mediated regulation of the stem cell state downstream of the patterning gene SCARECROW.
AB - The maintenance of stem cells in defined locations is crucial for all multicellular organisms. Although intrinsic factors and signals for stem cell fate have been identified in several species, it has remained unclear how these connect to the ability to reenter the cell cycle that is one of the defining properties of stem cells. We show that local reduction of expression of the RETINOBLASTOMA-RELATED (RBR) gene in Arabidopsis roots increases the amount of stem cells without affecting cell cycle duration in mitotically active cells. Conversely, induced RBR overexpression dissipates stem cells prior to arresting other mitotic cells. Overexpression of D cyclins, KIP-related proteins, and E2F factors also affects root stem cell pool size, and genetic interactions suggest that these factors function in a canonical RBR pathway to regulate somatic stem cells. Expression analysis and genetic interactions position RBR-mediated regulation of the stem cell state downstream of the patterning gene SCARECROW.
UR - http://www.scopus.com/inward/record.url?scp=29244464678&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2005.09.042
DO - 10.1016/j.cell.2005.09.042
M3 - Article
C2 - 16377572
AN - SCOPUS:29244464678
SN - 0092-8674
VL - 123
SP - 1337
EP - 1349
JO - Cell
JF - Cell
IS - 7
ER -