TY - JOUR
T1 - The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis
AU - Jacob, Pierre
AU - Brisou, Gwilherm
AU - Dalmais, Marion
AU - Thévenin, Johanne
AU - van der Wal, Froukje
AU - Latrasse, David
AU - Suresh Devani, Ravi
AU - Benhamed, Moussa
AU - Dubreucq, Bertrand
AU - Boualem, Adnane
AU - Lepiniec, Loic
AU - Immink, Richard G. H.
AU - Hirt, Heribert
AU - Bendahmane, Abdelhafid
N1 - KAUST Repository Item: Exported on 2021-05-25
Acknowledgements: We thank P. Audigier for plant handling, and are grateful for the cell biology imaging platform and research facilities provided by the Institute of Plant-Science Paris-Saclay (IPS2).
PY - 2021/5/15
Y1 - 2021/5/15
N2 - HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.
AB - HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.
UR - http://hdl.handle.net/10754/669225
UR - https://www.mdpi.com/2073-4425/12/5/746
U2 - 10.3390/genes12050746
DO - 10.3390/genes12050746
M3 - Article
C2 - 34063415
SN - 2073-4425
VL - 12
SP - 746
JO - Genes
JF - Genes
IS - 5
ER -