Three-dimensional imaging of monogenoidean sclerites by laser scanning confocal fluorescence microscopy

Paolo Galll; Giovanni Strona; Anna Maria Villa; Francesca Benzoni; Stefani Fabrizio; Silvia Maria Doglia; Delane C. Kritsky

doi:10.1645/GE-3544RN.1

Three-dimensional imaging of monogenoidean sclerites by laser scanning confocal fluorescence microscopy

Paolo Galll, Giovanni Strona, Anna Maria Villa, Francesca Benzoni, Stefani Fabrizio, Silvia Maria Doglia, Delane C. Kritsky

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A nondestructive protocol for preparing specimens of Monogenoidea for both α-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy. © American Society of Parasitologists 2006.

Original language	English (US)
Pages (from-to)	395-399
Number of pages	5
Journal	Journal of Parasitology
Volume	92
Issue number	2
DOIs	https://doi.org/10.1645/GE-3544RN.1
State	Published - Apr 1 2006
Externally published	Yes

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Parasitology

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abstract = "A nondestructive protocol for preparing specimens of Monogenoidea for both α-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy. {\textcopyright} American Society of Parasitologists 2006.",

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AU - Galll, Paolo

AU - Strona, Giovanni

AU - Villa, Anna Maria

AU - Benzoni, Francesca

AU - Fabrizio, Stefani

AU - Doglia, Silvia Maria

AU - Kritsky, Delane C.

N1 - Generated from Scopus record by KAUST IRTS on 2023-09-25

PY - 2006/4/1

Y1 - 2006/4/1

N2 - A nondestructive protocol for preparing specimens of Monogenoidea for both α-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy. © American Society of Parasitologists 2006.

AB - A nondestructive protocol for preparing specimens of Monogenoidea for both α-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy. © American Society of Parasitologists 2006.

UR - http://www.bioone.org/doi/abs/10.1645/GE-3544RN.1

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DO - 10.1645/GE-3544RN.1

M3 - Article

SN - 0022-3395

VL - 92

SP - 395

EP - 399

JO - Journal of Parasitology

JF - Journal of Parasitology

IS - 2

ER -

Three-dimensional imaging of monogenoidean sclerites by laser scanning confocal fluorescence microscopy

Abstract

ASJC Scopus subject areas

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