Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain

Alka Saxena, Akiko Wagatsuma, Yukihiko Noro, Takenobu Kuji, Atsuko Asaka-Oba, Akira Watahiki, Cecile Gurnot, Michela Fagiolini, Takao K. Hensch, Piero Carninci*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nanoCAGE and RNA seq.

Original languageEnglish (US)
Pages (from-to)381-385
Number of pages5
JournalBioTechniques
Volume52
Issue number6
DOIs
StatePublished - Jun 2012
Externally publishedYes

Keywords

  • FACS
  • Nano CAGE
  • Parvalbumin
  • Pyramidal
  • RNA seq

ASJC Scopus subject areas

  • Biotechnology
  • General Biochemistry, Genetics and Molecular Biology

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