Ultrafast excited-state dynamics of the photoswitchable protein dronpa

Eduard Fron, Cristina Flors*, Gerd Schweitzer, Satoshi Habuchi, Hideaki Mizuno, Ryoko Ando, Frans C. De Schryver, Atsushi Miyawaki, Johan Hofkens

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

70 Scopus citations


Dronpa is a photoswitchable protein from the family of green fluorescent proteins (GFPs). Photoswitching involves forward and backward proton transfer between a bright deprotonated form (B) and a dark protonated form (A2). We have used femtosecond transient absorption spectroscopy to determine the kinetics of the first step of the photoconversion from the dark to the bright form, which we have measured as 4 ps. The 2-fold isotope effect on the kinetics of this process shows that excited-state proton transfer (ESPT) is involved in this step. It was also demonstrated that the acid-induced protonated form A1 and the photoconverted protonated form A2 are two distinct spectroscopic species.

Original languageEnglish (US)
Pages (from-to)4870-4871
Number of pages2
JournalJournal of the American Chemical Society
Issue number16
StatePublished - Apr 25 2007
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry


Dive into the research topics of 'Ultrafast excited-state dynamics of the photoswitchable protein dronpa'. Together they form a unique fingerprint.

Cite this