TY - JOUR
T1 - Urinary Tract Infections Impair Adult Hippocampal Neurogenesis
AU - Darwish, Batoul
AU - Chamaa, Farah
AU - Awada, Bassel
AU - Lawand, Nada
AU - Saadé, Nayef E
AU - Abou Fayad, Antoine G
AU - Abou-Kheir, Wassim
N1 - KAUST Repository Item: Exported on 2022-06-27
Acknowledgements: The authors would like to acknowledge the National Council for Scientific Research of Lebanon (CNRS-L) for granting a doctoral fellowship to Batoul Darwish. The fellowship was offered in collaboration with the American University of Beirut. All authors have consented to this acknowledgement. This work was partly supported by the Medical Practice Plan (MPP) (grant: MPP-WAK-18).
PY - 2022/6/9
Y1 - 2022/6/9
N2 - Previous studies have suggested a link between urinary tract infections (UTIs) and cognitive impairment. One possible contributing factor for UTI-induced cognitive changes that has not yet been investigated is a potential alteration in hippocampal neurogenesis. In this study, we aim to investigate the effect of UTI on brain plasticity by specifically examining alterations in neurogenesis. Adult male Sprague Dawley rats received an intra-urethral injection of an Escherichia coli (E. coli) clinical isolate (108 CFU/mL). We found that rats with a UTI (CFU/mL ≥ 105) had reduced proliferation of neural stem cells (NSCs) at an early time point post infection (day 4) and neurogenesis at a later time point (day 34). This was associated with the decreased expression in mRNA of BDNF, NGF, and FGF2, and elevated expression of IL-1β in the hippocampus at 6 h post infection, but with no changes in optical intensity of the microglia and astrocytes. In addition, infected rats spent less time exploring a novel arm in the Y-maze test. Treatment with an anti-inflammatory drug did not revert the effect on NSCs, while treatment with antibiotics further decreased the basal level of their proliferation. This study presents novel findings on the impact of urinary tract infections on hippocampal neurogenesis that could be correlated with cognitive impairment.
AB - Previous studies have suggested a link between urinary tract infections (UTIs) and cognitive impairment. One possible contributing factor for UTI-induced cognitive changes that has not yet been investigated is a potential alteration in hippocampal neurogenesis. In this study, we aim to investigate the effect of UTI on brain plasticity by specifically examining alterations in neurogenesis. Adult male Sprague Dawley rats received an intra-urethral injection of an Escherichia coli (E. coli) clinical isolate (108 CFU/mL). We found that rats with a UTI (CFU/mL ≥ 105) had reduced proliferation of neural stem cells (NSCs) at an early time point post infection (day 4) and neurogenesis at a later time point (day 34). This was associated with the decreased expression in mRNA of BDNF, NGF, and FGF2, and elevated expression of IL-1β in the hippocampus at 6 h post infection, but with no changes in optical intensity of the microglia and astrocytes. In addition, infected rats spent less time exploring a novel arm in the Y-maze test. Treatment with an anti-inflammatory drug did not revert the effect on NSCs, while treatment with antibiotics further decreased the basal level of their proliferation. This study presents novel findings on the impact of urinary tract infections on hippocampal neurogenesis that could be correlated with cognitive impairment.
UR - http://hdl.handle.net/10754/679332
UR - https://www.mdpi.com/2079-7737/11/6/891
U2 - 10.3390/biology11060891
DO - 10.3390/biology11060891
M3 - Article
C2 - 35741412
SN - 2079-7737
VL - 11
SP - 891
JO - Biology
JF - Biology
IS - 6
ER -