Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region

Meizhong Luo; Yeisoo Yu; Hyeran Kim; Dave Kudrna; Yuichiro Itoh; Robert J. Agate; Esther Melamed; José L. Goicoechea; Jayson Talag; Christopher Mueller; Wenming Wang; Jennifer Currie; Nicholas B. Sisneros; Rod A. Wing; Arthur P. Arnold

doi:10.1016/j.ygeno.2005.09.005

Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region

Meizhong Luo, Yeisoo Yu, Hyeran Kim, Dave Kudrna, Yuichiro Itoh, Robert J. Agate, Esther Melamed, José L. Goicoechea, Jayson Talag, Christopher Mueller, Wenming Wang, Jennifer Currie, Nicholas B. Sisneros, Rod A. Wing, Arthur P. Arnold

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome. © 2005 Elsevier Inc. All rights reserved.

Original language	English (US)
Journal	Genomics
Volume	87
Issue number	1
DOIs	https://doi.org/10.1016/j.ygeno.2005.09.005
State	Published - Jan 1 2006
Externally published	Yes

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10.1016/j.ygeno.2005.09.005

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title = "Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region",

abstract = "The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome. {\textcopyright} 2005 Elsevier Inc. All rights reserved.",

author = "Meizhong Luo and Yeisoo Yu and Hyeran Kim and Dave Kudrna and Yuichiro Itoh and Agate, {Robert J.} and Esther Melamed and Goicoechea, {Jos{\'e} L.} and Jayson Talag and Christopher Mueller and Wenming Wang and Jennifer Currie and Sisneros, {Nicholas B.} and Wing, {Rod A.} and Arnold, {Arthur P.}",

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doi = "10.1016/j.ygeno.2005.09.005",

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AU - Luo, Meizhong

AU - Yu, Yeisoo

AU - Kim, Hyeran

AU - Kudrna, Dave

AU - Itoh, Yuichiro

AU - Agate, Robert J.

AU - Melamed, Esther

AU - Goicoechea, José L.

AU - Talag, Jayson

AU - Mueller, Christopher

AU - Wang, Wenming

AU - Currie, Jennifer

AU - Sisneros, Nicholas B.

AU - Wing, Rod A.

AU - Arnold, Arthur P.

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N2 - The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome. © 2005 Elsevier Inc. All rights reserved.

AB - The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome. © 2005 Elsevier Inc. All rights reserved.

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JF - Genomics

IS - 1

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Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region

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