Xeno-free self-assembling peptide scaffolds for building 3D organotypic skin cultures

Yihua Loo, Andrew C.A. Wan, Charlotte Hauser, E. Birgitte Lane, Paula Benny

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Organotypic skin cultures represent in vitro models of skin which can be used for disease modeling, tissue engineering, and screening applications. Non-human collagen is currently the gold standard material used for the construction of the supporting matrix, however, its clinical applications are limited due to its xenogeneic origin. We have developed a novel peptide hydrogel-based skin construct that shows a pluristratified epidermis, basement membrane, and dermal compartment after 3 weeks of in vitro culture. Peptide-based constructs were compared to collagen-based constructs and stratification marker expression was histologically higher in peptide constructs than in collagen constructs. Transepithelial electrical resistance also showed mature barrier function in peptide constructs. This study presents a novel application of the self-assembling peptide hydrogel in a defined xeno-free in vitro system.
Original languageEnglish (US)
JournalFASEB BioAdvances
DOIs
StatePublished - Jun 11 2022

Fingerprint

Dive into the research topics of 'Xeno-free self-assembling peptide scaffolds for building 3D organotypic skin cultures'. Together they form a unique fingerprint.

Cite this