The cytotoxic activity and antimicrobial properties of crude extracts from Laurus nobilis were investigated. With the use of the organic solvents, methanol and ethanol,
crude extracts were obtained.
To determine the availability of active bio-compounds, an analysis using liquid chromatography was conducted.
The crude extract was also tested for
antimicrobial activity.
The disc diffusion method was used against the bacterium Escherichia coli.
The results showed a weak antimicrobial
activity against E. coli. For
cytotoxicity testing, the crude extract was
studied on four cell-lines: human breast
adenocarcinoma, human embryonic kidney, HeLa
(human cervical adenocarcinoma), and human
lung fibroblast.
From the alamarBlue® assay results, the extracts most potently affected the
cell-lines of human breast adenocarcinoma and human embryonic kidney.
Using the lactate dehydrogenase (LDH) assay,
an effect on human embryonic kidney was
most prominent.
With these findings, a suggestion that the crude extract of Laurus nobilis may have
antiproliferative properties is put forth,
with the possibility of this mechanism
being induction of apoptosis with the involvement of Nuclear Factor Kappa κB (NF
κB).
| Date of Award | May 2011 |
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| Original language | English (US) |
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| Awarding Institution | - Biological, Environmental Sciences and Engineering
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| Supervisor | Kenneth Paul Minneman (Supervisor) |
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