Over the past decades, many studies have been conducted to generate in vitro
tissue systems that help understanding tissue development and disease
progression. Hydrogel scaffolds have been frequently used in creating such
models. Self-assembling peptide hydrogels are functional in providing the cells a
scaffold that supports cell proliferation, however, organoid and lumen formation
remains a challenge. Hydrogels can be synthesized and modified based on the
essential physiological properties, which can be achieved by altering the
chemical composition of the initial material. Thus, in this study, we test the effect
of the laminin-derived IKVAV motif on ultrashort self-assembling peptide in
relation to cell proliferation and lumen formation in colorectal cancer stem cells.
Further, we test the printability of the modified peptide. The modification of
ultrashort peptide serves the purpose of providing signals to direct cell adhesion,
differentiation, and lumen formation. One particular combination of peptides
showed the formation of colorectal organoids containing lumen of outperforming
characteristics as compared to the others, also in 3D bioprinting.
| Date of Award | Nov 2021 |
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| Original language | English (US) |
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| Awarding Institution | - Biological, Environmental Sciences and Engineering
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| Supervisor | Charlotte Hauser (Supervisor) |
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- Peptides
- 3D bioprinting
- IKVAV