Generation of iPSCs isogenic control from GLP1-R -/- patient iPSCs using CRISPR Cas9 system

  • Hussain Alqahtani

Student thesis: Master's Thesis


In this study, we employed CRISPR-Cas9 technology to generate isogenic induced pluripotent stem cells (iPSCs) with a corrected GLP1-R gene, serving as a control to patient-derived iPSCs harboring GLP1-R mutations. We tested these isogenic iPSCs and their pluripotency potential by measuring the expression of two common pluripotency markers Tra-1-81 and Tra-1-60. Moreover, we performed quantitative PCR (qPCR) experiments to measure the transcription levels of the GLP1-R gene in isogenic and mutant iPSC clones. Our findings revealed significant differences in GLP1-R transcription levels and mRNA structures between the mutant and isogenic clones. These insights contribute to a better understanding of the mosaic nature of mutant iPSCs and the functional consequences of GLP1-R mutations.
Date of AwardJul 2023
Original languageEnglish (US)
Awarding Institution
  • Biological, Environmental Sciences and Engineering
SupervisorMo Li (Supervisor)

Cite this