Since
its
description
in
2008,
Chromera
velia
has
drawn
profound
interest
as
the
closest
free--living
photosynthetic
relative
of
apicomplexan
parasites
that
are
significant
pathogens,
causing
enormous
health
and
economic
problems.
There--
fore,
this
newly
described
species
holds
a
great
potential
to
understand
evolu--
tionary
basis
of
how
photosynthetic
algae
evolved
into
the
fully
pathogenic
Apicomplexa
and
how
their
common
ancestors
may
have
lived
before
they
evolved
into
obligate
parasites.
Hence,
the
aim
of
this
work
is
to
understand
how
C.
velia
function
and
respond
to
different
environmental
conditions.
This
study
aims
to
reveal
how
C.
velia
is
able
to
respond
to
environmental
perturbations
that
are
applied
individually
and
simultaneously
since,
studying
stress
factors
in
separation
fails
to
elucidate
complex
responses
to
multi
stress
factors
and
un--
derstanding
the
systemic
regulation
of
involved
genes.
To
extract
biologically
significant
information
and
to
identify
genes
involved
in
various
physiological
processes
under
variety
of
environmental
conditions
(i.e.
a
combination
of
vary--
ing
temperatures,
iron
availability,
and
salinity
in
the
growth
medium)
we
pre--
pared
strand
specific
RNA--seq
libraries
for
83
samples
in
diverse
environmental
conditions.
Here,
we
report
the
set
of
significantly
differentially
expressed
genes
as
a
re--
sponse
to
the
each
condition
and
their
combinations.
Several
interesting
up--
regulated
and
down--regulated
genes
were
found
and
their
functions
and
in--
volved
pathways
were
studied.
We
showed
that
the
profound
regulation
of
HSP20
proteins
is
significant
under
stress
conditions
and
hypothesized
that
the--
se
proteins
might
be
involved
in
their
movements.
| Date of Award | May 2014 |
|---|
| Original language | English (US) |
|---|
| Awarding Institution | - Biological, Environmental Sciences and Engineering
|
|---|
| Supervisor | Arnab Pain (Supervisor) |
|---|
- Chromera velia
- Apicomplexa
- RNA-seq
- Factorial experiment
- stress